Genome Editing Beyond CRISPR
Abstract:
Genome editing in nuclear, mitochondrial, and chloroplast DNA is broadly useful for biomedical research, medicine, and biotechnology. CRISPR systems, widely used for nuclear DNA editing, are not suitable for organellar DNA editing, due to the difficulty of guide RNA delivery into organelles. Mitochondrial DNA editing paves the way for disease modeling of mitochondrial genetic disorders in cell lines and animals, and can offer treatment options for these diseases with unmet medical needs. Likewise, chloroplast DNA editing in plants provides unique opportunities in plant biotechnology and agriculture, contributing to carbon neutralization. I will present novel tools for A-to-G and C-to-T base editing of organellar DNA in human cells, animals, and plants.
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