Synthetic small intestines for treating and studying intestinal disease
In recent years, it has become widely accepted that in vitro intestinal models enable improved studies of intestinal function in an ethical and well-controlled manner. Such research led to new insights into cellular growth and proliferation, drug absorption capabilities and host-microbial interactions. By using a combination of laser ablation and microfabrication techniques, we created a plastic template with accurate dimensions and concentrations of human small intestinal villi. Since that time we have employed techniques making use of intestinal organoids and enteroids from murine and human-sourced tissue. These models are intended to improve accuracy, reproducibility and experimental control. For this talk I will compare techniques for understanding small intestinal function.