GT (SIALYLTRANSFERASE AND FUCOSYLTRANSFERASE) MUTAGENESIS AND METABOLIC ENGINEERING FOR THE SYNTHESIS OF 3‘/6‘-SL AND 2‘/3-FL FROM HUMAN MILK

ABSTRACT:

Oligosachharides in human milk(HMOs) have various useful biological functions for infant and applications in industry. Among them, sialyllactose(3’/6‘-SL) and fucosyllactose(2‘/3-FL) draw our atttention. To produce these oligosaccharides in large quantity, economic supply of CMP-Neu5Ac, highly active sialyltransferase(ST) and fucosyltransferase(FT) are keys to the success of the process development.